INVESTIGATION OF CLASS 1 INTEGRONS WITH ANTIBIOTIC RESISTANCE GENES IN MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII STRAINS AND DETERMINATION OF PLANT EXTRACT EFFECTS ON MULTIDRUG-RESISTANT ISOLATES

Saral A., Cinemre S., Okumus F., AKAR Z., ÇOPUR ÇİÇEK A., Duzgun A. O.

FARMACIA, cilt.69, sa.5, ss.914-918, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.31925/farmacia.2021.5.13
  • Dergi Adı: FARMACIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, EMBASE, International Pharmaceutical Abstracts
  • Sayfa Sayıları: ss.914-918
  • Anahtar Kelimeler: MIC, plant extract, resistance, SPECTRUM BETA-LACTAMASES, ANTIBACTERIAL ACTIVITY
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

This study aimed to investigate the presence of resistance genes in multidrug-resistant A. baumannii isolates as well as to determine the antibacterial activity of selected plant extracts against isolates. 41 strains were isolated from various clinical samples. PCR tests were performed using the primers. Methanol was used as solvent for the preparation of the plant extracts. MIC values of the plant extracts were determined by the broth microdilution method. The bla(OXA-)(23), bla(CTX-M-1), bla(CTX-M-2), bla(GES) genes and Class 1 integrons were detected in five isolated strains. The lowest MIC value (2.25 mg/mL) was determined for the Echinacea putpurea extract, while the highest MIC value (50 mg/mL) was determined for the Morus alba extract. Determination of the antibacterial effect of plants extracts used in the study against A. baumannii isolates shows the importance of screening the antibacterial activity of plants in the fight against antibiotic resistance.