Inhibitory effects of herbal extracts on ACHEs purified from Ricania simulans

Kızıl D., Dinçer B.

4 th EURASIA BIOCHEMICAL APPROACHES & TECHNOLOGIES (EBAT) CONGRESS., Antalya, Türkiye, 3 - 06 Kasım 2022, ss.59

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.59
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Acetylcholinesterase (ACHE, EC3.1.1.7) plays an important role in neurotransmission by hydrolyzing the neurotransmitter acetylcholine into choline and acetate and is the target site of many insecticides.1 Between the various alternatives for insect control, a lot of natural plant products that are nonphytotoxic, biodegradable and ecofriendly engage the attention of scientists worldwide. 2 Purified the acetylcholinesterase from adults and nymphs of R. simulans using affinity chromatography edrophonium-Sepharose.3 The inhibition effects of olive leaf, walnut leaf, walnut green shell and sycamore leaf on R. simulans ACHEs were examined and the IC50 values of the inhibitors were calculated. 4 As a result, the IC50 values for adults were 20,3; 34.1; 42.3; 75.2 µg dry matter/mL, respectively and for nymphs 16,2; 58.3; 56.0; 78,5 µg dry matter/mL, respectively. It was determined that olive leaf extract showed a higher inhibition effect on ACHEs in both stages compared to other extracts. While other extracts were more effective on adults according to their IC50 values, it was determined that olive leaf extract was more effective on nymphs.